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Bio Fuel is a special type of fuel used only in the Bio-Generator from the Mekanism mod. It can be produced by processing most types of plants in the Crusher. The easiness of its production makes it a good energy source on early game stage.
One unit of bio fuel produces 5 seconds of operation in the Bio-Generator, in total 12 kJ. Bio fuel is also used in the crafting recipe for the Bio-Generator.